To investigate whether netropsin can selectively kill HMGA2-overexpressing cancer cells, both DLD-1-Vector and DLD-1-HMGA2 cells were treated with netropsin, and then cell viability was examined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. This evidence concerns the gene HMGA2 and cancer.