CSDE1 and autism: We initially targeted the coding region of CSDE1 using a modified single-molecule molecular inversion probe (smMIP) approach (Materials and Methods) among 4045 ASD probands from the Autism Clinical and Genetic Resources in China (ACGC) cohort and identified three de novo LGD variants (two nonsense and one canonical splice site) from two simplex quad families and one trio family (CC1.p1, CC2.p1, CC3.p1; Table 1 and Fig. 1).