To validate the reproducibility and accuracy of the RNA-Seq data, 10 DEGs (Plin2, Alox15, Nr1i2, Nr1d1, Cd36, Lpl, Wfs1, Cck, Pomc, and Th) associated with T2D or exercise were chosen for transcript analysis by qRT-PCR (Figs. 5A–5J). The gene discussed is ALOX15; the disease is type 2 diabetes mellitus.