Although 1222 target genes of miR-10b-5p were predicted and 48 significantly negatively correlated genes were obtained, only 5 overlapped genes, BIRC5, E2F2, KIF2C, FOXM1, and MCM5, were considered as potential key targets of miR-10b-5p in breast cancer for further analysis. This evidence concerns the gene E2F2 and breast cancer.