After subcutaneous administration, both PLGA:DDA/HspX/EsxS/MPLA and PLGA:DDA/HspX/EsxS/MPLA (booster) as well as PLGA:DDA/HspX/EsxS and PLGA:DDA/HspX/EsxS (booster) are likely good subunit vaccine against TB due to induce an effective IFN-γ and IgG2a immune responses with a low increase in IL-4 and IgG1. Here, IFNG is linked to tuberculosis.