C3 and neoplasm: Moreover, immunofluorescence and immunoblotting on the final day of treatment showed that the levels of TRIB3 (Fig. 6E,H,I), LC3II (Fig. 6F,J,K) and cleaved C3 (Fig. 6G,L,M) were enhanced in these tumours when they had been treated with GA, supporting the idea that this compound also induces autophagy-mediated cell death in vivo.