Since nine cancer types did not have mRNA level data available for matched normal tissues, we analyzed 25 cancer types and found that 15 had significantly higher PGK1 mRNA level in tumor tissues than in normal tissues and that PGK1 mRNA levels were increased by more than twofolds in 4 of the 15 cancer types, namely, esophageal carcinoma (ESCA), uterine corpus endometrioid carcinoma (UCEC), cervical and endocervical cancer (CESC), and lung squamous cell carcinoma (LUSC) (Fig. 1a). This evidence concerns the gene PGK1 and cancer.