There was also a non-novel locus in the PCLO (piccolo presynaptic cytomatrix protein) gene involved in synaptic vesicle trafficking that, while not genome-wide significant in the PGC and 23andMe meta-analysis (Wray et al., 2018) did reach genome-wide significance separately for MDD in single cohort (Mbarek et al., 2017) that is included in the PGC meta-analyses. This evidence concerns the gene PCLO and major depressive disorder.