125I‐antiTLR5 mAb was injected into 4T1 tumour model mice to evaluate specificity of targeted probe based on TLR5 expression in tumour models, and the results revealed much higher radiotracer retention in 4T1 (TLR5+) tumours than in 4T1 (TLR5−) tumours at all checking time‐points both ex vivo distribution and in vivo whole‐body phosphor‐autoradiography. This evidence concerns the gene TLR5 and neoplasm.