In block group with unlabelled anti‐TLR5 antibody pre‐treatment, 125I‐antiTLR5 mAb failed to target TLR5 in tumour‐bearing mice, no tumour radio image could be obtained, which revealed the specificity of the 125I‐antiTLR5 mAb imaging. The gene discussed is TLR5; the disease is neoplasm.