To further analyze the functional impact of BRCC3 mutations found in AML t(8;21)(q22;q22.1) and MDS patients, we cloned the two missense mutations R81G and R82H, located within the MPN + domain as well as D135E located at the end of the JAMM motif (Fig. 1b). The gene discussed is BRCC3; the disease is acute myeloid leukemia.