According to the functional annotations of the DEGs, 4 genes of interest, including NLU004874.1 (encoding NlST45, a sugar transporter), NLU014287.2 (encoding SRp54, a serine and arginine-rich protein), NLU006811.1 (encoding CYP6AY1, a cytochrome P450 protein), and NLU012128.1 (encoding CPR70, a cuticle protein) with significant lower transcript abundance in TN1-BPH (FPKM = 0), but higher in Mudgo-BPH (FPKM > 6) were selected to determine their functions by RNAi method. Here, SRP54 is linked to benign prostatic hyperplasia.