We examined the level and distribution of selected Wnt/β-catenin target genes across hepatocyte and non-hepatocyte clusters, focusing on genes that were significantly upregulated in male zebrafish HCC from our bulk RNA-seq analysis (jun, axin2, wif1, myca) (Table S6) and/or previously implicated in hepatocarcinogenesis (jun, axin2, mtor, glula, myca) (Adebayo Michael et al., 2019; Okabe et al., 2016; Seki et al., 2012; Villanueva et al., 2008). Here, JUN is linked to hepatocellular carcinoma.