We compared Wnt pathway activity in adult male and female Tg(fabp10a:pt-β-cat) zebrafish HCC (Evason et al., 2015) and non-transgenic control sibling livers at 6 mpf by crossing these zebrafish to the Wnt reporter line Tg(7xTCF-Xla.Siam: mCherry) (Moro et al., 2012). This evidence concerns the gene BTK and hepatocellular carcinoma.