The survival time of mice treated with Tf-LPs (25 days) was longer than that of mice treated with saline (20 days), free DOX (22 days) and LPs (24 days), this is because Tf could specifically bind to the Tf receptor on the surface of U87 cells and cell-penetrating peptide can facilitate transport across the BBB, further enhancing anti-glioma efficacy [4,20]. Here, TF is linked to glioma.