RUNX1 and precursor B-cell acute lymphoblastic leukemia: This emerged as a strong correlation in our MRD positive cases which predominantly showed gain of copies of several genes including LEF1, RUNX1, PAR1, NR3C2, PHF6, and CASP8AP2. Over-expression of LEF1 has been reported to predict unfavorable outcome and the standard-risk B-ALL patients with high LEF1 expression can possibly benefit from early treatment modifications and alternative molecular therapies, such as agents targeting the Wnt signaling pathway (35).