Similar to the results in mice with T1DM, we found that AKT and AS160 phosphorylations as well as GLUT4 translocation to the plasma membrane were significantly reduced in cardiac tissues of mice with T2DM in comparison with control mice (P < 0.05); however, these changes reverted on carvacrol treatment (Figures 8A–D, P < 0.05). Here, TBC1D4 is linked to type 2 diabetes mellitus.