Western blotting showed that levels of phosphorylated AKT and AKT substrates (molecular weight 160 kDa; AS160; Figures 7A, B) significantly decreased in the heart of mice with T1DM when compared with control mice; carvacrol treatment restored the levels of phosphorylated AKT and AS160. This evidence concerns the gene TBC1D4 and type 1 diabetes mellitus.