Additionally, we assessed hypothalamic protein levels of Ionized calcium-binding adapter molecule 1 (IBA1), a marker for microglial activation and promotion of reactive gliosis, that has been associated with diet-induced obesity and hypothalamic dysfunction in mice with a just recently described role in enhancing orexigenic AgRP expression upon insulin action (Valdearcos et al., 2017; Winkler et al., 2019). Here, AIF1 is linked to Obesity.