All reactions were normalized on the beta‐actin (actB) level as this HKG showed stable expression with small variance in our samples (Ct = 23.1 ± 0.3 (n = 6) in control vs 22.8 ± 0.7 (n = 5) in HE, P = .7, Student's t test). Here, ACTB is linked to hereditary elliptocytosis.