To verify whether TXNDC12 induced EMT in HCC cells through upregulation of ZEB1, we knocked down ZEB1 expression in TXNDC12-overexpressing cells and observed that ZEB1 knockdown eliminated the TXNDC12-mediated upregulation of Vimentin and downregulation of E-cadherin (Fig. 3d). The gene discussed is VIM; the disease is hepatocellular carcinoma.