The result revealed that CRHBP group significantly increase the protein levels of p-AKT, p-p65 and p-IkBα, as well as decrease the expression of IkBα compared with vector group both in 769P and ACHN cells (Fig. 5c–f), indicating that CRHBP may be capable of promoting ccRCC inflammation in part via activating the NF-kB signaling pathway. The gene discussed is NFKBIA; the disease is nonpapillary renal cell carcinoma.