To assess whether our results were specific to the U138 genetic background or whether MAP4K4 acts as a more general regulator of glioblastoma migration we tested the effect of a small-molecule inhibitor of MAP4K4 (PF 06260933 dihydrochloride) on migration in three additional adult glioma lines (LN18, U87-MG, T98G). Here, MAP4K4 is linked to glioblastoma.