Furthermore, in fast progressing DN and HCC of E2f1 transgenic mice, MYBL2, Clusterin, Cdc2, and Cyclin B1 expression was higher than in the lesions of c-Myc transgenic mice, and anti- MYBL2 siRNA had the highest anti-proliferative and apoptogenic effects in cell lines from HCC of E2f1 transgenic mice. This evidence concerns the gene CLU and hepatocellular carcinoma.