The combination of clinical and biochemical findings including abdominal pain/distension, pruritus, jaundice, and weight loss, imaging techniques like ultrasound (US), computed tomography (CT), magnetic resonance imaging (MRI), 18F-fluorodeoxyglucose positron emission tomography (18FDG-PET), and serum analysis of non-specific tumor biomarkers such as CA199, CEA (carcinoembryonic antigen), and CD133 [180], usually help to diagnose CCA, but ultimately liver biopsy and pathological diagnosis are used as the gold standard [181]. The gene discussed is CEACAM5; the disease is cholangiocarcinoma.