To do this, monocytes were latently infected with a second recombinant TB40E isolate expressing mCherry, which allows latently infected cells to be identified for much longer times after infection and overcomes the waning of GFP expression, which is known to occur when using a GFP-tagged virus (Krishna et al., 2016), and then stained these cells for HCLS1. The gene discussed is HCLS1; the disease is infection.