Interestingly, using the same system for afadin labeling but using anti‐JAM‐A as a first antibody, we could not detect JAM‐A in the pedestals nor associated with ZO‐1 in these structures at 4 hr of infection of L or MDCK cells (Figure A2), which was different than during the TJ formation, where JAM‐A is associated with ZO‐1 after its dissociation from afadin (Ooshio et al., 2010). This evidence concerns the gene AFDN and infection.