On the other hand, the infection with the mutant EPECΔespF caused a drastic decrease in afadin recruitment at every infection time points, in comparison with EPEC‐infected cells, with very low values of 5 pixels per pedestal at 45 min of infection reaching a maximum of 50 pixels per pedestal at 4 hr of infection, which could be considered background levels of detection (Figure 3u). The gene discussed is AFDN; the disease is infection.