F11R and infection: Interestingly, using the same system for afadin labeling but using anti‐JAM‐A as a first antibody, we could not detect JAM‐A in the pedestals nor associated with ZO‐1 in these structures at 4 hr of infection of L or MDCK cells (Figure A2), which was different than during the TJ formation, where JAM‐A is associated with ZO‐1 after its dissociation from afadin (Ooshio et al., 2010).