At 3 hr of infection, the colocalization signal between afadin (Figure 3m) and ZO‐1 (Figure 3n) began to disappear as individual signals started to segregate inside of the largest pedestals (Figure 3o), while in the pedestals of smaller size, the afadin–ZO‐1 colocalization remained constant, or only ZO‐1 was detected (Figure 3p, O, and N). This evidence concerns the gene AFDN and infection.