AFDN and infection: Notably, at 3 and 4 hr of infection, the afadin signal remained undetectable in the pedestal structures (Figure A3i, m) while the recruited ZO‐1 signal decorated the small actin pedestals (Figure A3j–l and n–p) but with less signal intensity than that observed in wild‐type EPEC‐induced pedestals (Figure A3j, n).