At 2 hr of infection, increasing ZO‐1 signal was detected in these cumuli in the pedestal structures (Figure 3j), and surprisingly, afadin was abundantly detected in the actin pedestals (Figure 3i), strongly colocalizing with ZO‐1 in these structures (Figure 3k), but only in the highest pedestals and not in the smaller one. This evidence concerns the gene AFDN and infection.