AFDN and infection: At 6 hr of infection, both ZO‐1 and afadin were recruited in every pedestal formed at the edge of the monolayer with different maturation states: In some pedestals, there was colocalization; in other pedestals, both proteins were in the process of delocalization; and in lesser number, the stem and tip pattern was observed for ZO‐1 and afadin, respectively (Figure 11c).