At 2 hr of EPEC‐induced infection, the pedestals that were formed at the edge of the monolayer had already initiated ZO‐1 recruitment (Figure 11a), while those formed at the cell–cell contacts were erected at sites where ZO‐1 is assembled in the intercellular junctions, meanwhile displaying a similar pedestal size (0.4 μm) for both populations (Figure 11d). This evidence concerns the gene TJP1 and infection.