To assess the polyfunctionality of DNV‐responsive T cells we assessed the ability of T cells to produce multiple cytokines or their ability to carry out certain effector functions by determining the frequency of 15 different combinations of IFNγ, TNF‐α, CD107a, and MIP‐1β production by DENV–NS3‐ (Figure 2A) and DENV–NS5 (Figure 2B)‐specific CD8+ T cells in patients with DF and DHF. This evidence concerns the gene CD8A and dengue disease.