The cell death phenotype of shCRT-transduced cells was further confirmed using viability fluorescent staining and quantitative flow cytometric analysis, revealing a 4- to 7-fold increase of Zombie/AnnexinV -positive cells compared with shCont transduced counterparts in multiple independent patient-derived melanoma cells (Figs 1G and 1H and S1E). The gene discussed is ANXA5; the disease is melanoma.