CALR and cancer: To independently validate our findings that CRT down-regulation leads to the significant increase of mitochondrial Ca2+ levels, we used Human cervical adenocarcinoma cell line (HeLa) cancer cell-line expressing genetically encoded calcium indicator 3, created from a fusion of green fluorescent protein, calmodulin, and M13, a peptide sequence from myosin light chain kinase (GFP-CaMP3).