We found that ZFAS1 was up‐regulated in most of our preserved CCA cells, and after ZFAS1 silencing in CCLP‐1 and RBE cells, the CCK‐8 assay, colony formation, migration and invasion abilities all significantly glided downside but apoptosis by Hoechst 33342 and caspase‐3. This evidence concerns the gene ZFAS1 and cholangiocarcinoma.