As one of our previous works shows that a larger number of studies have indicated that the ZFAS1 is overexpressed in many human cancers, such as breast cancer, gastric cancer, colorectal cancer hepatocellular cancer and many others, which is one important evidence to imply the potential role of ZFAS1 in the progression of human tumours.33 So that, we performed a series of experiments to detect whether ZFAS1 could act as one important factor in CCA. This evidence concerns the gene ZFAS1 and neoplasm.