As an example, correlation between gene amplification at global level by qualitative polymerase chain reaction (qPCR) and expression by IF is reported to be a challenging item, and we found it inconsistent in brain tumors.17 This finding has a key impact on the therapeutic approach, so it’s worth to emphasize on an EGF/VEGF chimer characterized with an adequate amount of antibodies in contradiction of the EGF and VEGF function which aimed to prevent angiogenic and growth of breast tumor in mouse model.18 The gene discussed is VEGFA; the disease is breast neoplasm.