EGF and brain neoplasm: As an example, correlation between gene amplification at global level by qualitative polymerase chain reaction (qPCR) and expression by IF is reported to be a challenging item, and we found it inconsistent in brain tumors.17 This finding has a key impact on the therapeutic approach, so it’s worth to emphasize on an EGF/VEGF chimer characterized with an adequate amount of antibodies in contradiction of the EGF and VEGF function which aimed to prevent angiogenic and growth of breast tumor in mouse model.18