Gene methylation correlates with low transcript expression, and Aza-C demethylation treatment restores AXIN2 expression in a dose-dependent fashion in CRC cell lines.21 Interestingly, epigenetic suppression of two key Wnt NRs (AXIN2, NKD1) in LD tumours can occur independently of the CpG island methylator phenotype, where promoter methylation is globally dysregulated.22 This suggests that targeted, rather than stochastic methylation of Wnt regulatory genes may occur in these lesions. The gene discussed is NKD1; the disease is colorectal carcinoma.