ANXA5 and cancer: To investigate the apoptosis induction in MCF-7 and MDA-MB-231 cancer cells, both DoHuRu-hosting POPC and DOTAP formulations were analysed by fluorescence-activated cell sorting (FACS) analysis by using Annexin V-FITC (fluorescein isothiocyanate) along with the propidium iodide (PI) dye, a very sensitive method to differentiate apoptotic (Annexin V-FITC positive, PI negative) from necrotic (Annexin V-FITC positive, PI positive) cells [162].