Thus, SWATH‐MS–based quantitative proteomic analysis has been used to analyse glycoproteins secreted by a colon adenocarcinoma cell line that were confirmed by immunodetection 23 or to determinate the molecular effects induced by DDHD1 silencing in colorectal cancer cells.24 Therefore, SWATH‐MS studies are particularly useful for better proteome characterization and may accelerate future discovery and validation of CRC biomarkers. This evidence concerns the gene DDHD1 and colorectal carcinoma.