Ishikawa cells are a well‐differentiated endometrial adenocarcinoma cell line that retains the endometrial epithelial phenotype and displays apical adhesiveness and expression profiles of different molecules similar to those of the endometrium under the control of oestradiol and progesterone.42, 43 To investigate the relationship between β‐catenin and Snail in E2‐induced EMT, Ishikawa cells were transfected with β‐catenin siRNA and Snail siRNA for 24 hours and were incubated with E2 for another 24 hours. Here, SNAI1 is linked to endometrium adenocarcinoma.