In the current study, we detected the expression of G3BP1, p‐AKT, and YB1 proteins in 48 cases of noncancerous control lung tissues and 247 cases of NSCLC by IHC and investigate the mRNA level of G3BP1 and YB1, and thereby exploring the relationship between the expression of G3BP1, YB1, and p‐AKT proteins and clinicopathological features and their prognostic significance in NSCLC. The gene discussed is YBX1; the disease is non-small cell lung carcinoma.