To explore the potential biological molecular mechanism that might mediate the upregulation of HAI-1 in HCC, firstly, we cloned four HAI-1 promoter fragments upstream of mRNA start site (mRNA sequence: GenBank AB000095) from -4036 bp to mRNA start site (designated as position 0), illustrated in Figure 2(a), named B, C, D, and E clones further matched or enzyme digested into seven fragments (Figure 2(b)). This evidence concerns the gene SPINT1 and hepatocellular carcinoma.