We also further confirmed that CD133− cancer cells exposure to the HMGB1+ irradiated cells or rhHMGB1 led to acquisition of higher migration ability (Fig. S2B) and mesenchymal phenotype accompanied by up-regulation of mesenchymal makers (N-cadherin and Vimentin) and down-regulation of epithelial marker (E-cadherin) (Fig. 2b and Fig. S3A). Here, HMGB1 is linked to cancer.