When the least absolute shrinkage and selection operator (LASSO) with .632+ bootstrap was applied on LC/MRM-MS measurements of eleven protein biomarkers on the discovery set, a four-marker algorithm consisting of MASP1, OPN, PON3, and TR yielded AUCsBS of 0.80, 0.75, and 0.84 for all, early, and late stage CRC detection, respectively. This evidence concerns the gene MASP1 and colorectal carcinoma.