SPP1 and colorectal carcinoma: When the least absolute shrinkage and selection operator (LASSO) with .632+ bootstrap was applied on LC/MRM-MS measurements of eleven protein biomarkers on the discovery set, a four-marker algorithm consisting of MASP1, OPN, PON3, and TR yielded AUCsBS of 0.80, 0.75, and 0.84 for all, early, and late stage CRC detection, respectively.