Feeding mice a maternal methyl supplemented diet causes gut dysbiosis and associated changes in colonic mucosal DNA methylation and transcriptomic patterns, and colitis in resulting offspring (121).The presence of gut bacteria stimulates hypermethylation of CpG motifs in the Tlr4/TLR4 gene, whereas in the percentage of methylation of CpG motifs in the 5′ region of the Tlr4 gene becomes reduced within the large intestine of GF mice (122). This evidence concerns the gene TLR4 and colitis.