ABL1 and chronic myelogenous leukemia, BCR-ABL1 positive: The scenario of the new molecular technologies employed in CML has been recently enriched by the Next Generation Sequencing (NGS); this method is now frequently used for detecting ABL1 kinase domain (KD) mutations, that are one relevant mechanism of CML resistance to TKIs and that account for ~50% of acquired resistance in failing CML cases (69).