Therefore, this study unraveled the richness and abundance of the microbial community of colorectal tumor samples in comparison to healthy mucosa through 16S rRNA gene sequencing in a small cohort of patients treated at Barretos Cancer Hospital, and correlated different microbial phylotypes with clinicopathologic features and molecular characteristics (such as tumor location, BRAF mutation, and MSI status). This evidence concerns the gene BRAF and neoplasm.