GSK3B and hepatocellular carcinoma: Figures 4D–H shows that GSK3β inhibitor significantly attenuated the ARG-induced depression of β-catenin signaling in HCC. In addition, Hep G2 and SMMC 7721 cells were incubated with or without ARG for 24 h, lysates were immune-precipitated with GSK3β, and western blotting analysis for β-catenin and GSK3β showed that ARG treatment enhanced of the combination between GSK3β and β-catenin (Figures 4I–K).