As there have been conflicting results regarding sphingolipids in PD brains (Cheng et al., 2011; Fabelo and Martin, 2011; Abbott et al., 2014; Murphy et al., 2014; Gegg et al., 2015; Bienias et al., 2016), we wanted to investigate whether inhibiting nSMase2 affects the lipid composition of sphingolipids after the addition of oα-syn in the absence or presence of hypoxia using UPLC-MS/MS. This evidence concerns the gene SMPD3 and Parkinson disease.