NLRP3 and obesity due to melanocortin 4 receptor deficiency: Furthermore, activation of the Nlrp3 inflammasome in Wt C57BL/6 or Nlrp1b1-Tg bone marrow derived macrophages in response to ATP (Fig. 3A) or danger signals associated with obesity like glucose (Fig. 3B) or palmitic acid (Fig. 3C) resulted in similar levels of IL-1β secreted to the culture supernatant.