To identify the mechanisms through which HGF and its cognate receptor, c-MET, induces EMT in bladder cancer we treated the rat bladder carcinoma cell line NBT-II, with either HGF or EGF and analysed changes in cellular morphology with EMT marker expression (Fig. 1a, Supplementary Fig. 1a). This evidence concerns the gene EGF and urinary bladder carcinoma.