PRKG1 and ischemia: In this study, in hearts pretreated with NO donor NOC-18 before ischemia, we observed changes in phosphorylation level of mitochondrial membrane-bound and matrix proteins, however, the protein phosphorylation pattern was not replicated by treatment of mitochondria with isolated PKG suggesting that mitochondrial proteins involved in fatty acid cycle and oxidative phosphorylation complexes might be modified indirectly through other NO-mediated pathways, e.g., involving cytosolic PKC [18] or ROS signaling.