To assess HR efficiency, the activated Cas9-HSPCs were electroporated with either sgLmnb1 or sgActb and subsequently infected with AAV-DJ vectors carrying the corresponding donor templates for Lmnb1 (AAV-DJ-Lmnb1) or Actb (AAV-DJ-Actb) at a multiplicity of infection (MOI) of 5 × 106 virus genome copies (GCs) per cell (Figure 1B). Here, ACTB is linked to infection.