Upregulated MIEN1 suppressed the expression of MMP9 by PKB/Akt expression downregulation. About 80% increase in MIEN 1 expression in evaluation to ordinary ANTS. MIEN 1 level was completely interrelated with age, tumor grade, metastasis of the patient and negatively correlated with overall survival of the patient. The gene discussed is MMP9; the disease is neoplasm.