The top 10 significant gene aberrantly expressed in NAFLD included five up-regulated genes [ENO3 (P = 7.17E-05), CYP7A1 (P = 9.04E-05), FMO1 (P = 6.57E-04), PEG10 (P = 8.95E-04), and MAMDC4 (P = 1.87E-03)] and five down-regulated genes [P4HA1 (1.67E-04), CYP1A1 (P = 2.51E-04), IGFBP2 (P = 3.27E-04), SOCS2 (P = 5.1E-04), and SHBG (P = 1.50E-03)]. The gene discussed is SHBG; the disease is metabolic dysfunction-associated steatotic liver disease.