To further confirm whether the altered expression of these 10 genes (NFKBIβ, EGFR, FOS, NFKB1, PPARα, RXRα, RXRβ, TPMT, UGCG, and XPC) in DLD-1 colon cancer cells would affect their responsiveness towards chemotherapeutics agents, MTT assay was performed to analyze the viability of the transfected cells after etoposide treatment. The gene discussed is PPARA; the disease is colonic neoplasm.