In current research, the expression level of XPC gene was downregulated in DLD-1 colon cancer cells with the deficient of both BAX and BAK. Therefore, to verify if the upregulation of cellular energy metabolism and the drug resistance observed in DLD-1 BAX–BAK DKO cells was associated with the suppression of XPC gene, seahorse analysis was used to assess the ECAR and OCR in DLD-1 siXPC cells and DLD-1 WT cells. The gene discussed is BAK1; the disease is malignant colon neoplasm.