While the data obtained employing the aging and OVX models only suggested that estrogen plays a role in hypertension in rodents, in the studies by the Milner group, the use of the AOF model uniquely allowed the testing of the hypothesis that irregular estrogen fluctuations during AOF transition, rather than loss of estrogen at post-AOF, may be responsible for the observed increase in susceptibility to AngII-induced hypertension. This evidence concerns the gene AGT and hypertensive disorder.