Based on the in vivo animal model of developmental seizures induced by lithium chloride-pilocarpine, this study explored the long-term effects of zinc deficiency and zinc supplementation on developmental seizure-induced brain damage, focusing on the parameters of cognition, seizure threshold, hippocampal regenerative mossy fiber sprouting and expression of ZnT-3 and GPR39 in hippocampus to further reveal the relationship between zinc and epileptogenesis and provide new insights for the prevention and treatment of epilepsy. The gene discussed is GPR39; the disease is Zinc deficiency.